5/29/2023 0 Comments Introduction to light microscopyIn addition, high-resolution three-dimensional (3D) imaging of small, whole organisms is now feasible across time. ![]() Recent progress in light microscopy allows for unprecedented insights into nanostructures, as well as unprecedented experimental throughput. With regard to systems biology, microscopy is a tool that connects multiple scales of biological complexity, ranging from molecules to populations. ![]() Recent progress in biotechnology, technology, and interdisciplinary cooperation provides more realistic insights into biological processes than ever before. Current genomic tools in combination with advances in microscopy and computation facilitate in vivo observations of any genetic entity of interest. The main challenge of the post-genomic era is understanding the rules governing dynamic biological systems. However, understanding living dynamic biological systems by examining fixed specimens is, at the best, a heuristic process. For many decades, chemical fixation and the slicing of biological matter have been used to improve the stability and optical properties of samples. However, light scattering and other optical properties of living matter complicate the acquisition of informative images. One of the ultimate goals of systems biology is to elucidate relationships between molecular system states and higher order phenotypic traits. Not surprisingly, visualization techniques are at the heart of science and engineering. Most of our sensory neocortex is engaged in the processing of visual inputs that we gather from our surroundings.
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